The 6775 Freezer/Mill® is a small cryogenic mill that is designed for grinding small samples in the range of 0.1 – 5 grams. It is specifically designed to cryogenically grind tough and/or temperature sensitive samples. The mill cools samples to cryogenic temperatures, then pulverizes them by magnetically shuttling a steel impactor back and forth against two stationary end-plugs. Since the vial is closed, the integrity of its contents is maintained, hazardous or critical samples are easily controlled, cleanup is simplified and cross-sample contamination is eliminated.

The new high resolution display is user-friendly and includes the following features:

Run history which is uploadable to a thumb drive via USB

Training videos and manuals that can be viewed on the screen.

A complete listing of accessories

Applications include: DNA/RNA extraction, THC extraction, protein extraction, forensic testing, materials testing, pharmaceutical analysis, drug testing, RoHS/WEEE and volatiles extraction.

Typical samples include: Animal tissue, plastic and polymers, yeast, pharmaceuticals, food products, electronic components, textiles, hair, teeth, bones, plant tissue such as cannabis, seeds, leaves and roots.

Applications include: DNA/RNA extraction, THC extraction, protein extraction, forensic testing, materials testing, pharmaceutical analysis, drug testing, RoHS/WEEE and volatiles extraction.

Typical samples include: Animal tissue, plastic and polymers, yeast, pharmaceuticals, food products, electronic components, textiles, hair, teeth, bones, plant tissue such as cannabis, seeds, leaves and roots.

0105-154503-CPSC phthalates in plastics guidelines updated.pdf - CPSC phthalates in plastics guidelines

0626-165623-FM Yeas Flyer 2018.pdf - Freezer/Mill - DNA and Protein extractions from Yeast cultures

1019-144346-HT003-FM-How-to-RoHs_WEEE.pdf - The use of Cryogenic grinding to comply with the RoHS/WEEE Directive

1019-143552-HT001 - FM - How to - Guidelines for Cryogenic Grinding of Bone-2016.pdf - How To Guidelines - Grinding of Bone in Freezer/Mill

0308-113156-SP007-FM-Grinding_Polymers_for_Qualitative_Quantitative_Analysis.pdf - Grinding Polymers for Qualitative Analysis

0504-164013-SP028_FM_Trace_metal_analysis_Pet_Food_app_note.pdf - Trace Metal Analysis of Commercial Pet Food for Toxic Metals by ICP and ICP-MS

0614-114701-Cryogenic Milling for high throughput residue sample preparation_IUPAC2014.pdf - Cryogenic Milling for high throughput residue sample preparation_IUPAC2014

0621-164516-Forensic-prep_flyer_2018.pdf - Forensics Sample Preperation

0621-120309-Plastic-Polymer-prep_flyer_2018.pdf - Polymers & Plastics Sample Preparation

0627-142736-SP002 - FM - Prep of Domestic Waste for Elemental Analysis.pdf - Preparation of Domestic Waste for Elemental Analysis

1019-154735-SP005 - FM - RNA Extraction from Cartilage Tissue.pdf - RNA Extraction from Cartilage Tissue Using Cryogenic Grinding

0627-142109-SP006 - FM - Preparation of Rice Husks for Analysis.pdf - Preparation of Rice Husks for the Analysis of Their Contents

1020-102003-SP008 - FM - Structural Changes in Polymers_v2.pdf - Structural Changes in Polymers by Means of Cryogenic Grinding

0627-141838-SP009 - FM - Nafion-Powder for Production of Fuel Cell Electrodes.pdf - Nafion® - Powder for the Production of Fuel Cell Electrodes

0627-105354-SP015 - FM Molecular Effects of Polution in Cetacean Samples.pdf - Molecular Effects of Pollution in Cetacean Samples Using the Cytochrome P450, CYP 1A1, 1A2, and 1B1 as Biomarkers

Brochures

Pharmaceutical Sample Preparation 6775

All Freezer/Mill Brochure

Freezer/Mill CRYOGENIC GRINDING FOR PLASTICS AND POLYMERS

Freezer/Mill CRYOGENIC GRINDER FOR FORENSIC APPLICATIONS

6755 Freezer/Mill Cryogenic Grinder

Freezer/Mill SAMPLE PREPARATION FOR POLYMERS & PLASTICS LABS

Insulated liquid nitrogen tub is equipped with LN level sensor.

Lid safety interlock for operator protection.

Grinding Vial Sets offered in a variety of materials including polycarbonate, stainless steel, and Cr-free steel.

Grinding chamber holds one small grinding vial or one microvial set.

Touch screen stores up to 20 user-defined grinding programs for easy quick recall. Also includes run history, training videos, manuals and accessories.

Dimensions 19 in (48cm) x 10.5 in (27cm) x 12.75 in (32cm)

Voltage 115V/60Hz or 230V/50Hz

Net Weight (lbs) 18.9 lbs (8.6kg)

Gross Weight (lbs) 26 lbs (11.8kg)

Power Cord 3-prong grounded plug, 115V 60Hz or 2-prong European plug, 230V50Hz

Timer/Controls Touch-screen programmable controls for grinding cycles, time, impact rate, pre-cooling and cooling times. Stores 20 grinding programs.

CE Approved Yes

Vials And Vial Sets

6751 (6751) SMALL GRINDING VIAL SET View Details

6751C20 (6751C20) SMALL POLYCARBONATE CENTER CYLINDER View Details

6751C4 (6751C4) SMALL POLYCARBONATE CENTER CYLINDER PACK OF 4 View Details

6751E (6751E) SMALL STAINLESS STEEL END PLUGS View Details

6751P (6751P) SMALL STAINLESS STEEL IMPACTOR View Details

6757 (6757) MICROVIAL SET View Details

6757C3 (6757C3) MICROVIAL CYLINDER View Details

6757P (6757P) MICROVIAL IMPACTOR View Details

6759 (6759) MICROVIAL HOLDER View Details

6761 (6761) SMALL POLY-VIAL SET View Details

6761P (6761P) SMALL POLYCARBONATE ENCAPSULATED IMPACTOR View Details

6771 (6771) SMALL CR-FREE GRINDING VIAL SET View Details

6771E (6771E) SMALL CR-FREE STEEL END PLUGS View Details

6771P (6771P) SMALL CR-FREE STEEL IMPACTOR View Details

6781E 6781E REPLACEMENT END PLUGS FOR 6781S View Details

6781O 6781O O-RINGS FOR 6781S View Details

6801 (6801) LARGE GRINDING VIAL SET View Details

6801C20 (6801C20) LARGE POLYCARBONATE CENTER CYLINDER

PACK OF 20 View Details

6801C4 (6801C4) LARGE POLYCARBONATE CENTER CYLINDER - PACK OF 4 View Details

6801E (6801E) LARGE STAINLESS STEEL END PLUGS View Details

6801P (6801P) LARGE STAINLESS STEEL IMPACTOR View Details

6802 (6802) LARGE STAINLESS STEEL CENTER CYLINDER View Details

6803 (6803) LARGE STAINLESS STEEL VIAL SET View Details

6870S (6870S) GRINDING VIAL ACCESSORY PACKAGE View Details

6871 (6871) LARGE CR-FREE GRINDING VIAL SET View Details

6871E (6871E) LARGE CR-FREE STEEL END PLUGS View Details

6871P (6871P) LARGE CR-FREE STEEL IMPACTOR View Details

6881 (6881) MID-SIZE GRINDING VIAL SET View Details

6881C20 (6881C20) MID-SIZED POLYCARBONATE CENTER CYLINDER PACK OF 20 View Details

6881C4 (6881C4) MID-SIZE POLYCARBONATE CENTER CYLINDERS View Details

6881E (6881E) MID-SIZE STAINLESS STEEL END PLUGS View Details

6881P (6881P) MID-SIZE STAINLESS STEEL IMPACTOR View Details

6883 (6883) MID-SIZE CR-FREE GRINDING VIAL SET View Details

6883E (6883E) MID-SIZE CR-FREE STEEL END PLUGS View Details

6883P (6883P) MID-SIZE CR-FREE STEEL IMPACTOR View Details

6885 (6885) MID-SIZE POLY-VIAL SET View Details

6885E (6885E) MID-SIZE POLYCARBONATE END PLUGS View Details

6885P (6885P) MID-SIZE POLYCARBONATE ENCAPSULATED IMPACTOR View Details

Racks & Holders

6755 (6755) VIAL RACK FOR SMALL GRINDING VIALS View Details

6805 (6805) VIAL RACK FOR LARGE GRINDING VIALS View Details

Extractors

6753C (6753C) SAMPLE EXTRACTION TOOL View Details

6754 (6754) SMALL EXTRACTOR/VIAL OPENER View Details

6756 (6756) EXTRACTOR FOR SMALL VIALS View Details

6758 (6758) EXTRACTOR FOR MICROVIALS View Details

6791 6791 - VIAL IMPACTOR EXTRACTOR FOR FREEZER/MILL VIALS View Details

6804 (6804) LARGE EXTRACTOR/VIAL OPENER View Details

6808 (6808) LARGE VIAL EXTRACTOR View Details

Sample Adapters

6807 (6807) MULTI-VIAL HOLDER FOR SMALL & MICRO VIALS View Details

6808M (6808M) MID-SIZE VIAL ADAPTER KIT View Details

6870M-1 (6870M-1) MID-SIZE VIAL ADAPTER PACKAGE View Details

6887 (6887) MID-SIZED VIAL ADAPTER View Details

6888 (6888) MID-SIZE VIAL ADAPTER-CLOSED END View Details

Accessory Packages & Kits

6870L (6870L) LARGE GRINDING VIAL ACCESSORY PACKAGE View Details

Liquid Nitrogen Handling

54006 (54006) DIFFUSER View Details

6900L (6900L) CRYOGENIC GLOVES, SIZE LARGE View Details

6900M (6900M) CRYOGENIC GLOVES, SIZE MEDIUM View Details

6900S (6900S) CRYOGENIC GLOVES, SIZE SMALL View Details

6900XL (6900XL) CRYOGENIC GLOVES, SIZE EXTRA LARGE View Details

6906 (6906) SHORT CRYOGENIC TRANSFER HOSE, 4 FT (1.2 M) View Details

6907 (6907) LONG CRYOGENIC TRANSFER HOSE, 6 FT (1.8M) View Details

Tissue Homogenization and Cell Lysis

What are the applications for the Geno/Grinder®?

The SPEX SamplePrep 2010 Geno/Grinder® was originally designed as a titer plate shaker for extracting DNA from seeds and plant tissue. This involved shaking two sealed, 96-well titer plates with plant tissue, buffer, and a steel grinding ball in each well. Over the years, the versatility of the Geno/Grinder design has enabled it to accommodate a wider variety of sample containers and sample types. Not only can it be used for high-throughput tissue homogenization, DNA/RNA and protein extraction, it can also be used for lysing bacteria and yeast. Most recently it has been used to increase sample throughput for pesticide residue analysis using the QuEChERS method. A wide range of Application Notes are available which discuss the various Geno/Grinder® applications in more detail.

What are the advantages of the Geno/Grinder® over other tissue homogenizers?

Unlike other tissue homogenizers which clamp titer-plates on their side and move in a "figure-eight motion", the 2010 Geno/Grinder® uses a vertical shaking motion - a much more efficient grinding process. This ensures that the grinding media impacts the sample each and every time and ultimately results in higher yields. Another benefit of the vertical grinding action is that titer-plates and vials are kept in their upright position, eliminating potential well-to-well contamination. The Geno/Grinder is also able to accommodate a larger quantity and variety of sample formats including micro-centrifuge and PCR tubes, titer-plates, vial sets, and conical bottom centrifuge tubes. The list of Geno/Grinder accessories has been greatly expanded making it possible to grind a much wider range of samples. Cryo-Blocks® and related Kryo-Tech® accessories preserve temperature sensitive samples and can be used for extracting RNA and proteins from both plant and animal tissue.

What are the benefits of grinding at low temperatures?

Most samples become embrittled at low temperatures, making them easier to grind. When working with nucleic acids, grinding at low temperatures can also result in higher yields.

What type of samples can I process in the Geno/Grinder®?

The 2010 Geno/Grinder® can be used for a variety of samples including plant tissues like soybeans, sugar beet leaves, fungi, and seeds. It also grinds animal tissues like liver and fat and can lyse yeast and bacterial cells.

Grinding and Pulverizing

How to I choose a laboratory mill?

If your samples can be pulverized by impact at room temperature, you have a choice of several proven SPEX SamplePrep laboratory mills. The 8530 Enclosed Shatterbox® and 8500 Shatterbox® are ring-and-puck mills that are ideal for rapid grinding of samples up to approximately 100 grams, in 5 minutes or less. The 8000M Mixer/Mill® and 8000D Dual Mixer/Mill® are high-energy ball mills that not only pulverize samples in the 10 gram range but are suitable for blending powders and making emulsions up to approximately 50 mL. The 5100 Mixer/Mill® is a smaller high-energy ball mill, with about 10% the sample capacity of the 8000-series Mixer/Mills. All these mills have multiple-sample capacity with smaller samples, and a wide choice of grinding container sizes and materials, for maximum on-the-job flexibility over a wide rangeof applications. Soft, brittle materials and flexible samples that can be ground by shearing as well as impact may be suitable for the 5200 Micro Hammer-Cutter Mill, which uses revolving hammers in a chamber with a serrated lining. Product particle size is controlled by perforated screens in the bottom of the grinding chamber. This is the only "flow-through" mill sold by SPEX SamplePrep, and has proven useful for a comparatively narrow range of samples. The 6770 Freezer/Mill® and 6870 Freezer/Mill® are unique cryogenic mills that can grind almost any "ungrindable" sample, including all plant and animal tissue, most polymers, and countless other samples that are resistant to room-temperature milling. Freezer/Mills require liquid nitrogen for chilling the sample, and maintaining cryogenic temperatures in the vial during grinding. Forty years after its introduction the Freezer/Mill remains the most widely used and most effective cryogenic mill in the world. Finally, the 2010 Geno/Grinder® is a high-throughput tissue homogenizer with an adjustable clamp that accommodates a variety of formats ranging from deep-well titer plates to centrifuge tubes. It is specifically designed for rapid cell disruption, lysis, and tissue homogenization while preserving temperature sensitive samples. To discuss any SPEX SamplePrep laboratory mills and their suitability for your applications, feel free to contact our product specialists. We also offer test-grinding of your samples in our applications lab, or we can loan you a mill for short-term trials in your own lab. The more you know about SPEX SamplePrep laboratory mills, the more

How have the Freezer/Mills® been used in the field of medical research?

Medical schools and research hospitals have long used Freezer/ Mill technology in a variety of unconventional projects. One is the pulverizing of fresh, sterilized bone to use as a cement in joint-replacement surgery. The tiny bone fragments act as growth nuclei to stimulate the formation of bone around prosthetic implants. Another is the homogenizing of heart tissue to smear on slides as a culture medium. Freezer/Mills have even been used to grind implant materials to a fine powder which can be injected into live subjects for allergy and toxicity testing. At dental schools cryogenic grinding is used to pulverize fresh teeth without degrading them through heating. The overall possibilities in the medical field seem endless. A wide range of Application Notes which discuss the various Freezer/Mill® applications in more detail are available.