The 1600 MiniG® is the ideal solution for labs that want a compact yet powerful tissue homogenizer and cell lyser. It is equipped with an adjustable clamp that accommodates a full range of sample vials from 2mL to 50mL centrifuge tubes or up to two deep-well titer plates. It is specifically designed for rapid cell disruption, cell lysis and tissue homogenization through bead beating, enabling fast and efficient extraction of nucleic acids, proteins and other molecules of interest.

Applications include: Tissue Homogenization, DNA/RNA Research and extraction, Cell Lysis, Protein Extraction, Biofuel research, Pesticide Residue extraction using QuEChERS or the SWeET method.

Typical samples include: Animal tissue, Plant Tissue, Cell cultures, Fruit, Cannabis, Seeds, Yeast and Bacteria.

0316-133753-SP024-GG-Pesticide Analysis - QuEChERS and Improved Methods.pdf - Analysis of Pesticides in Fruit and Vegetable Products Using the Standard QuEChERS Method and Improved Methods involving Geno/Grinder

0325-090155-SP027 - MiniG_Stable isotope ratio analysis for food origin origin_FoodForensics2015.pdf - Analysis of stable isotope ratios for the verification of authenticity of food sources and production methods

QuEChERS & LC-MS / MS (UCT) Quantitative Analysis of Whole Blood Benzodiazepin

0702-093825-5101-02 QuEChERS in blood provided by QuEChERS.pdf for extraction of whole blood anabolic steroids (UCT)

0714-161459-5105-01 Neonicotinoids in honey--DA-UHPLC.pdf - Analysis of neonicotinoids in honey by QuEChERS and UHPLC-MS/MS (UCT)

0714-162825-4106-03 QuEChERS.pdf Pesticides in Soil - Determination of Pesticide Residues in Soil Using the QuEChERS Method (UCT)

0714-163129-4109-01 Mycotoxins in Cereals LC-MS-MS.pdf - Determination of Mycotoxin Residues by LC-MS/MS with Two Alternative Sample Extraction Methods (UCT)

0714-164054-5102-02 Cannabinoids in Cannabis and Edibles Provided by QuEChERS.pdf - QuEChERS (UCT) Extracts Cannabinoids in Cannabis and Edibles

0714-164643 - Determination of chlorophenoxyacetic acid and other acidic herbicides using the QuEChERS sample preparation method and LC-MS_MS analysis.pdf - Determination of chlorophenoxyacetic acid and other acids using the QuEChERS sample preparation method and LC-MS/MS analysis (UCT) herbicide

0714-165645 - Malachite green in seafood - LC-MS.pdf - Simple SPE method for the determination of malachite green, crystal violet and other synthetic dyes in seafood, combined with LC-MS/MS detection (UCT)

0714-165810-pesticide_residue_analysis_in_whole_milk.pdf - Analysis of pesticide residues in whole milk by QuEChERS and LC-MS/MS (UCT)

1218-111913-Pharma-Prep_Flyer.pdf - Drug Sample Preparation

0613-170036 - Cryogenic Milling for High Throughput Residue Sample Preparation_IUPAC2014.pdf - Cryogenic Milling for High Throughput Residual Sample Preparation_IUPAC2014

0118-162623-Bead_beating_Intro_guide_3.pdf - Bead Beating Guide

0402-163004-8101-03-01-Pigmentation_Removal_in_Cannabis.pdf - ChloroFiltr® dSPE Clean-up and LC-MS/MS remove purple pigmentation from cannabis using QuEChERS extraction

Determination of 11-nor-9-Carboxy-THC in Urine by QuEChERS and LC-MS/MS (UCT)

0714-164525-Avocado_Pesticides.pdf - Analysis of 136 pesticides in avocado using the modified QuEChERS method and LC-MS/MS and GC-MS/MS (UCT).

0415-162823 - Marijuana Pesticide Residues and Marijuana Efficacy.pdf - Determination of Pesticide Residues and Marijuana Potency in Cannabis and Hemp Foods

0621-160451-Agri-prep_flyer_2018.pdf - Agricultural Sample Preparation

0714-164344-5104-04 QuEChERS.pdf - Determination of organochlorine pesticides in serum - Determination of organochlorine pesticides (UCT) in serum by an improved QuEChERS method

0714-164755-DeterminationofofBisphenol_A_in_Beverages_by_QuEChERS.pdf - Determination of Bisphenol A in Beverages by QuEChERS and LC-MS/MS (UCT)

0714-165449-Ephedra alkaloids Application Note-2.pdf - Strong cation exchange SPE and LC-MS/MS detection using pentafluoropropyl-based HPLC (UCT) - Determination of ephedra alkaloids and synephrine

1019-145358-SP001 - GG - Cell Disruption - Fatty Acid Extraction from Animal Tissue Samples.pdf - Mechanical Fragmentation of High-Throughput Fatty Acids from Animal Tissue Samples

SP011.pdf - Extraction of Nucleic Acid from Sugar Beet Leaves

SP013.pdf - Perchloric extract leaves for measuring starch and soluble metabolites

SP014.pdf - RNA Extraction from Aspergillus Parasitic Mycelia

SP017.pdf - Effect of Lysis Time and Other Variables on Fresh Basil Extracted DNA Solubilized with Geno/Grinder® in a 2 ml Test Tube

0415-160501-SP018 - GG - High-throughput disruption yeast in 96-well format.pdf - High-throughput disruption yeast in 96-well format

SP019.pdf - Comparison of methods for isolation of soybean DNA

1010-155815-SP020 - GG - Bacterial Cell Lysis _ Update.pdf - Genomic DNA Splitting in Geno / Grinder

SP021.pdf - Extraction of RNA/cDNA and genomic DNA from tissues using real-time PCR

SP022.pdf - Geno/Grinder® High-Throughput Tissue Homogenizer Improves LC/MS/MS Pesticide Residue Analysis Sample Flux Advantages

0921-105606-SP023-GG - Comparison of pesticide extraction in pesticides_2016.pdf - Comparison of pesticide extraction in agricultural products using manual shaking and mechanical mixing

0714-163743-5104-01 QuEChERS.pdf Pesticides in coffee - Determination of pesticides in coffee using QuEChERS extraction and silica gel SPE purification (UCT)

SP025.pdf - Rapid extraction of DNA from rice seeds (wet)

Brochures

MiniG QuEChERS English

MiniG Homogenizer

Pharmaceutical Sample Preparation MiniG

Automated mechanical disruption through bead beating, ideal for high-throughput cell lysis applications including DNA/ RNA extraction, protein extraction and pesticide residue analysis.

Window in lid allows you to see the grinding process.

Clamp typically holds up to two titer plates or 48 x 2 mL vials or 48 x 5 mL vials when stacked.

Clamp also accommodates taller vials such as 12 x 15 mL or 6 x 50 mL falcon tubes.

Lid has a safety interlock switch that stops the motor if the lid is opened during the grinding process.

Optional Kryo-Tech® Accessories enable cryogenic grinding and preserve temperature sensitive samples for RNA and protein extractions.

LCD display screen shows run times in minute : second format.

Rate & timer controls allow you to adjust speed and set time.

Start and stop buttons allow you to start and stop the grinding process.

Voltage 115V / 60Hz or 230V / 50Hz

CE Approved Yes

Dimensions 21.5 in. (54.6 cm) high x 11.25 in. (28.6 cm) wide x 17.0 in. (43.2 cm) deep.

Weight 40lbs. (18 kg)

Motor 1/7 HP

Power Cord 3-prong grounded plug-115V / 60Hz.2-prong european cord for 230V / 50Hz.

Clamp Movement 1.25 in. (3.2 cm) vertical

Clamp Speed Adjustable from 500 to 1500 strokes/minute

Titer Plates & Cap Mats

2200 (2200) 96 - WELL TITER PLATE, SQUARE WELLS View Details

2200-100 (2200-100) 96 - WELL TITER PLATE, SQUARE WELLS, PACK OF 100 View Details

2201 (2201) CAP-MAT FOR 2200 TITER PLATE View Details

2201-10 (2201-10) CAP-MAT FOR 2200 TITER PLATE, PACK OF 10 View Details

2210 (2210) 96 - WELL TITER PLATE, ROUND WELLS View Details

2210-100 (2210-100) 96 - WELL TITER PLATE, ROUND WELLS, PACK OF 100 View Details

2211 (2211) CAP-MAT FOR 2210 TITER PLATE View Details

2211-10 (2211-10) CAP-MAT FOR 2210 TITER PLATE, PACK OF 10 View Details

2220-100 (2220-100) 48 - WELL TITER PLATE, RECTANGULAR WELLS, PACK OF 100 View Details

2221 (2221) CAP-MAT FOR 2220 TITER PLATE View Details

2221-10 (2221-10) CAP-MAT FOR 2220 TITER PLATE, PACK OF 10 View Details

2230 (2230) 24 - WELL TITER PLATE, SQUARE WELLS View Details

2230-100 (2230-100) 24 - WELL TITER PLATE, SQUARE WELLS, PACK OF 100 View Details

2231 (2231) CAP-MAT FOR 2230 TITER PLATE View Details

2231-10 (2231-10) CAP-MAT FOR 2230 TITER PLATE, PACK OF 10 View Details

Vials And Vial Sets

1681 (1681) 5ML BLOCK View Details

2240-PC (2240-PC) PRE-CLEANED 5 ML POLYCARBONATE VIAL SET View Details

2241-PC (2241-PC) 5 ML POLYCARBONATE VIAL WITH SCREW-ON CAP View Details

2250 (2250) PRE-CLEANED 15 ML SHORT POLYCARBONATE VIAL SET View Details

2251-PC (2251-PC) 15 ML SHORT POLYCARBONATE VIAL WITH SCREW-ON CAP View Details

2251C (2251C) CAPS FOR 15ML SHORT POLYCARBONATE VIAL View Details

2252-PC-30 (2252-PC-30) 15 ML POLYCARBONATE VIAL View Details

2253-PC-48 (2253-PC-48) 50ML POLYCARBONATE VIALS, 48 PK View Details

2300-500E (2300-500E) POLYPROPYLENE 2ML SKIRTED DISRUPTION TUBES AND CAPS View Details

2301-100MB (2301-100MB) PRE-FILLED 2ML TUBES W/ 100UM SILICA BEADS View Details

2302-1000AW (2302-1000AW) PRE-FILLED 2ML TUBES W/1.0MM ZIRCONIUM BEADS View Details

2302-1400AW (2302-1400AW) PRE-FILLED 2ML TUBES W/ 1.4 MM ZIRCONIUM BEADS View Details

2302-1700AW (2302-1700AW) PRE-FILLED 2ML TUBES W/ 1.7 MM ZIRCONIUM BEADS View Details

2302-200AW (2302-200AW) PRE-FILLED 2ML TUBES W/ 200UM ZIRCONIUM BEADS View Details

2302-3000AW (2302-3000AW) PRE-FILLED 2ML TUBES W/ 3.0 MM ZIRCONIUM BEADS View Details

2302-6000AW (2302-6000AW) 2ML PRE-FILLED VIALS - 6MM CERIA STABILIZED ZIRCONIA BEADS View Details

2303-MM1 (2303-MM1) PRE-FILLED 2ML TUBES WITH 500 UM GARNET AND A 6 MM ZIRCONIUM GRINDING SATELLITE View Details

2303-MM2 (2303-MM2) PRE-FILLED 2ML TUBES WITH 800 UM AND 1.4 MM ACID WASHED ZIRCONIUM BEADS View Details

2303-MM3 (2303-MM3) PRE-FILLED 2ML TUBES WITH 100 UM SILICA BEADS, 1.4 MM ZIRCONIUM BEADS AND A 4 MM SILICA BEAD, ACID WASHED View Details

2304-100AW (2304-100AW) PRE-FILLED 2ML TUBES W/ 100 UM SILICA BEADS (600MG) View Details

2304-400AW (2304-400AW) PRE-FILLED 2ML TUBES W/ 400UM SILICA BEADS FOR BACTERIA APPLICATIONS View Details

2304-800AW (2304-800AW) PRE-FILLED 2ML TUBES W/

800UM SILICA BEADS View Details

2305-2800SS (2305-2800SS) PRE-FILLED 2ML TUBES W/ 2.8 MM S/S GRINDING BALLS View Details

2310 (2310) 2ML DURATUBE View Details

3116PC (3116PC) POLYCARBONATE VIAL WITH SLIP-ON CAP View Details

Grinding Media

2100 (2100) GRINDING BALL DISPENSER View Details

2110M (2110M) 24-PIN MAGNET View Details

2110M-12 (2110M-12) PACK OF 12 MAGNETIC TIPS View Details

2110M-96 (2110M-96) PACK OF 96 MAGNETIC TIPS View Details

2110S (2110S) SEPARATOR, 24-PIN MAGNET View Details

2150 (2150) STAINLESS STEEL GRINDING BALLS, 5/32 IN. View Details

2155 (2155) STAINLESS STEEL GRINDING BALLS, 3/8IN View Details

2156 (2156) STAINLESS STEEL GRINDING BALLS, 7/16 IN. View Details

2160 (2160) SILICA GRINDING BEADS (800-1000 UM) View Details

2161 (2161) SILICA GRINDING BEADS - MOLECULAR BIOLOGY GRADE View Details

2162 (2162) LOW BINDING SILICA GRINDING BEADS (800 UM) View Details

2165 (2165) SILICA GRINDING BEADS (400-600 UM) View Details

2166 (2166) SILICA GRINDING BEADS - MOLECULAR BIOLOGY GRADE View Details

2167 (2167) LOW BINDING SILICA GRINDING BEADS (400 UM) View Details

2168 (2168) LOW BINDING SILICA GRINDING BEADS (100 UM) View Details

2180 (2180) ZIRCONIUM GRINDING BEADS - MOLECULAR BIOLOGY GRADE View Details

2181 (2181) LOW BINDING ZIRCONIUM GRINDING BEADS View Details

2182 (2182) LOW BINDING ZIRCONIUM GRINDING BEADS View Details

2186 2186 - ZIRCONIA GRINDING BEADS, 6MM View Details

Kryo-Tech®

1660 (1660) 2 ML CRYOBLOCK FOR MINIG View Details

1665 (1665) 5 ML CRYOBLOCK FOR MINIG View Details

1666 (1666) CRYO-BLOCK FOR 15 ML CENTRIFUGE TUBES View Details

1667 (1667) CRYO-BLOCK FOR 50 ML CENTRIFUGE TUBES View Details

1668 (1668) CRYO-BLOCK FOR 15 ML POLYCARBONATE VIALS View Details

2600 (2600) CRYO-STATION View Details

2650 (2650) CRYO-ADAPTER FOR 2210 TITER PLATES View Details

Racks & Holders

1680 (1680) 2ML FOAM BLOCK View Details

1685 (1685) 15 ML FOAM HOLDER FOR MINIG View Details

1686 (1686) 50 ML FOAM HOLDER FOR MINIG View Details

1690T (1690T) NESTING PLATE View Details

Kryo-Tech®

1690 (1690) ADJUSTABLE CLAMP View Details

1691 (1691) MINIG CLAMP LID View Details

Tissue Homogenization and Cell Lysis

What are the applications for the Geno/Grinder®?

The SPEX SamplePrep 2010 Geno/Grinder® was originally designed as a titer plate shaker for extracting DNA from seeds and plant tissue. This involved shaking two sealed, 96-well titer plates with plant tissue, buffer, and a steel grinding ball in each well. Over the years, the versatility of the Geno/Grinder design has enabled it to accommodate a wider variety of sample containers and sample types. Not only can it be used for high-throughput tissue homogenization, DNA/RNA and protein extraction, it can also be used for lysing bacteria and yeast. Most recently it has been used to increase sample throughput for pesticide residue analysis using the QuEChERS method. A wide range of Application Notes are available which discuss the various Geno/Grinder® applications in more detail.

What are the advantages of the Geno/Grinder® over other tissue homogenizers?

Unlike other tissue homogenizers which clamp titer-plates on their side and move in a "figure-eight motion", the 2010 Geno/Grinder® uses a vertical shaking motion - a much more efficient grinding process. This ensures that the grinding media impacts the sample each and every time and ultimately results in higher yields. Another benefit of the vertical grinding action is that titer-plates and vials are kept in their upright position, eliminating potential well-to-well contamination. The Geno/Grinder is also able to accommodate a larger quantity and variety of sample formats including micro-centrifuge and PCR tubes, titer-plates, vial sets, and conical bottom centrifuge tubes. The list of Geno/Grinder accessories has been greatly expanded making it possible to grind a much wider range of samples. Cryo-Blocks® and related Kryo-Tech® accessories preserve temperature sensitive samples and can be used for extracting RNA and proteins from both plant and animal tissue.

What are the benefits of grinding at low temperatures?

Most samples become embrittled at low temperatures, making them easier to grind. When working with nucleic acids, grinding at low temperatures can also result in higher yields.

What type of samples can I process in the Geno/Grinder®?

The 2010 Geno/Grinder® can be used for a variety of samples including plant tissues like soybeans, sugar beet leaves, fungi, and seeds. It also grinds animal tissues like liver and fat and can lyse yeast and bacterial cells.

Grinding and Pulverizing

How to I choose a laboratory mill?

If your samples can be pulverized by impact at room temperature, you have a choice of several proven SPEX SamplePrep laboratory mills. The 8530 Enclosed Shatterbox® and 8500 Shatterbox® are ring-and-puck mills that are ideal for rapid grinding of samples up to approximately 100 grams, in 5 minutes or less. The 8000M Mixer/Mill® and 8000D Dual Mixer/Mill® are high-energy ball mills that not only pulverize samples in the 10 gram range but are suitable for blending powders and making emulsions up to approximately 50 mL. The 5100 Mixer/Mill® is a smaller high-energy ball mill, with about 10% the sample capacity of the 8000-series Mixer/Mills. All these mills have multiple-sample capacity with smaller samples, and a wide choice of grinding container sizes and materials, for maximum on-the-job flexibility over a wide rangeof applications. Soft, brittle materials and flexible samples that can be ground by shearing as well as impact may be suitable for the 5200 Micro Hammer-Cutter Mill, which uses revolving hammers in a chamber with a serrated lining. Product particle size is controlled by perforated screens in the bottom of the grinding chamber. This is the only "flow-through" mill sold by SPEX SamplePrep, and has proven useful for a comparatively narrow range of samples. The 6770 Freezer/Mill® and 6870 Freezer/Mill® are unique cryogenic mills that can grind almost any "ungrindable" sample, including all plant and animal tissue, most polymers, and countless other samples that are resistant to room-temperature milling. Freezer/Mills require liquid nitrogen for chilling the sample, and maintaining cryogenic temperatures in the vial during grinding. Forty years after its introduction the Freezer/Mill remains the most widely used and most effective cryogenic mill in the world. Finally, the 2010 Geno/Grinder® is a high-throughput tissue homogenizer with an adjustable clamp that accommodates a variety of formats ranging from deep-well titer plates to centrifuge tubes. It is specifically designed for rapid cell disruption, lysis, and tissue homogenization while preserving temperature sensitive samples. To discuss any SPEX SamplePrep laboratory mills and their suitability for your applications, feel free to contact our product specialists. We also offer test-grinding of your samples in our applications lab, or we can loan you a mill for short-term trials in your own lab. The more you know about SPEX SamplePrep laboratory mills, the more

How have the Freezer/Mills® been used in the field of medical research?

Medical schools and research hospitals have long used Freezer/ Mill technology in a variety of unconventional projects. One is the pulverizing of fresh, sterilized bone to use as a cement in joint-replacement surgery. The tiny bone fragments act as growth nuclei to stimulate the formation of bone around prosthetic implants. Another is the homogenizing of heart tissue to smear on slides as a culture medium. Freezer/Mills have even been used to grind implant materials to a fine powder which can be injected into live subjects for allergy and toxicity testing. At dental schools cryogenic grinding is used to pulverize fresh teeth without degrading them through heating. The overall possibilities in the medical field seem endless. A wide range of Application Notes which discuss the various Freezer/Mill® applications in more detail are available.