The Geno/Grinder® is an automated high-throughput plant & animal tissue homogenizer and cell lyser.  It is equipped with an adjustable clamp that accommodates a full range of sample vials from 2mL to 50mL centrifuge tubes or up to six deep-well titer plates.  It is specifically designed for rapid cell disruption, cell lysis and tissue homogenization through bead beating.  This enables fast and efficient extraction of nucleic acids, proteins and other molecules.  The new model Geno/Grinder incorporates a password protected, touch screen control panel, enabling the user to program run time, rate, cycles and pause time.

A full range of Kryotech® accessories are available to preserve temperature sensitive samples such as proteins and RNA.  The Geno/Grinder is also recognized as an excellent automatic axial extractor.  It is the ideal tool for the QuEChERS method used for extracting pesticide residues and other organic compounds.

The Geno/Grinder enables increased throughput, improved extraction efficiency and reproducibility over traditional sample preparation methods. A full range of pre-loaded sample vials for different sample types is also available for fast and simple set up.

Applications include: Tissue Homogenization, DNA/RNA Research and extraction, Cell Lysis, Pesticide Residue Extraction, Protein Extraction, Biofuel research and QuEChERS.

Typical samples include: Animal tissue, Plant Tissue, Cell cultures, Fruit, Cannabis, Seeds, Yeast and Bacteria.

0626-170214-SP024 - GG - Pesticide Analysis - QuEChERS vs. Modified Method 2018.pdf - Analysis of Pesticides in Fruit and Vegetable Products using a Standard QuEChERS Method and a Modified Method Involving the Geno/Grinder

0325-090155-SP027 - MiniG_Stable Isotope Ratio Analysis for the testing of food product origins_FoodForensics2015.pdf - Stable Isotope Ratio Analysis for the testing of authenticity of food product origin and production method claims

0702-093341-4111-01 Benzos in blood by QuEChERS.pdf - Quantitative Analysis of Benzodiazepines in whole blood by QuEChERS & LC-MS/MS (UCT)

0702-093825-5101-02 Steroids in blood by QuEChERS.pdf - The application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood (UCT)

0714-161459-5105-01 Neonicotinoids in honey - DA -UHPLC.pdf - Analysis of Neonicotinoids in Honey by QuEChERS and UHPLC-MS/MS (UCT)

0714-162825-4106-03 Pesticides in soil by QuEChERS.pdf - Using a QuEChERS Approach for the Determination of Pesticide Residues in Soil (UCT)

0714-163129-4109-01 mycotoxins in grains by LC-MS-MS.pdf - Determination of Mycotoxin Residues by LC-MS/MS featuring Two Alternate Sample Extraction Procedures (UCT)

0714-164054-5102-02 Cannabinoids in Marijuana and Edibles by QuEChERS.pdf - Extraction of Cannabinoids in Marijuana and Edibles by QuEChERS (UCT)

0714-164643-Determination of Chlorophenoxyacetic Acid and Other Acidic Herbicides Using a QuEChERS Sample Preparation Approach and LC-MS_MS Analysis.pdf - Determination of Chlorophenoxyacetic Acid and Other Acidic Herbicides

Using a QuEChERS Sample Preparation Approach and LC-MS/MS Analysis (UCT)

0714-165645-Malachite Green in Seafood - LC-MS.pdf - A Simple SPE Method for the Determination of Malachite Green, Crystal Violet and Other Synthetic Dyes in Seafood Coupled with LC-MS/MS Detection (UCT)

0714-165810-pesticide_residue_analysis_in_whole_milk.pdf - Pesticide Residue Analysis in Whole Milk by QuEChERS and LC-MS/MS (UCT)

0626-164631-Pharma-prep_flyer_2018.pdf - Pharmaceutical Sample Preparation

0613-170036-Cryogenic Milling for high throughput residue sample preparation_IUPAC2014.pdf - Cryogenic Milling for high throughput residue sample preparation_IUPAC2014

0118-162623-Bead_beating_Intro_guide_3.pdf - Bead Beating Guide

0402-163004-8101-03-01-Pigmentation_Removal_in_Cannabis.pdf - Removal of Purple Pigmentation from Cannabis using QuEChERS Extraction, ChloroFiltr® dSPE Clean-up and LC-MS/MS

0627-143709-SP013 - GG - Perchloric Acid Extraction of leaves 2018.pdf - Perchloric Acid Extraction of Leaves for Measurement of Starch and Soluble Metabolites

0423-163207-5102-01 thca in urine by quechers.pdf - Determination of 11-nor-9-Carboxy-THC in Human Urine by QuEChERS and LC-MS/MS (UCT)

0714-164525-Avocado_Pesticides.pdf - Analysis of 136 pesticides in avocado using a modified QuEChERS method with LC-MS/MS and GC-MS/MS (UCT)

0415-162823-Pesticide Residues and Cannabis Potency in Marijuana.pdf - Determination of Pesticides Residues and Cannabis Potency in Marijuana and Cannabis Foods

0621-160451-Agri-prep_flyer_2018.pdf - Agriculture Sample Preparation

0627-101223-SP021_revised_2018 .pdf - Extraction of RNA/cDNA and Genomic DNA from Tissue with Real-Time PCR

0714-164344-5104-04 Organochlorine pesticides in serum by QuEChERS.pdf - A Modified QuEChERS Method for the Determination of Organochlorine Pesticides in Serum (UCT)

0714-164755-Determination_of_Bisphenol_A_in_Beverages_by_QuEChERS.pdf - Determination of Bisphenol A in Beverages by QuEChERS and LC-MS/MS (UCT)

0714-165449-Ephedra alkaloids application note -2.pdf - Determination of Ephedra Alkaloids & Synephrine by Strong Cation-Exchange SPE and LC-MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column (UCT)

0627-142944-SP001 - GG - Cell disruption 2018.pdf - Mechanical Disruption for High-Throughput Fatty Acid Extraction from Animal Tissue Samples

0627-141605-SP011 - GG - Extraction of Nucleic Acid from Sugar Beet Leaves 2018.pdf - Extraction of Nucleic Acids from Sugar Beet Leaves

0627-110251-SP013 - GG - Perchloric Acid Extraction of leaves - Starch & Soluable Metabolites 2018.pdf - Perchloric Acid Extraction of Leaves for Measurement of Starch and Soluble Metabolites

0627-105507-SP014 - GG - RNA Extraction from Pspergillus Parasiticus Myceluim 2018.pdf - RNA Extraction from Aspergillus parasiticus mycelium

0627-104633-SP017 - GG - Lysis, Time and other Variables - DNA Extraction from Basil 2018.pdf - Effect of Lysis Time and Other Variables on DNA Extraction from Fresh Basil Lysed in 2 ml Tubes with the Geno/Grinder®

0627-104425-SP018 - GG - High Throughput Disruption of Yeast in a 96-well format 2018.pdf - High Throughput Disruption of Yeast in a 96-Well Format

0627-102105-SP019 - GG - Comparasion of Methods for the Isolation of DNA from Soybeans 2018.pdf - Comparison of Methods for the Isolation of DNA from Soybeans

0627-101409-SP020 - GG - Lysing of Bacterial Cells_Updated 2018.pdf - Lysing of bacterial cells in the Geno/Grinder

0627-092240-SP022 - GG - Benefits of the GG for Pesticide Residue Analysis 2018.pdf - The Benefits of the Geno/Grinder® High-Throughput Tissue Homogenizer to Increase Sample Throughput for Pesticide Residue Analysis by LC/MS/MS

0627-091900-SP023 APP NOTE.pdf - Comparison of Pesticide Extraction in Agricultural Products Using a Manual Shaking Method and Mechanical Mixing with the Geno/Grinder

0714-163743-5104-01 Pesticides in coffee by QuEChERS.pdf - Determination of Pesticides in Coffee with QuEChERS Extraction and Silica Gel SPE Cleanup (UCT)

0626-170831-SP025_Revised_2016_Quick DNA Extraction from Rice Seed (Wet) 2018.pdf - Quick DNA Extraction from Rice Seed (Wet)

Automated mechanical disruption through bead beating, ideal for high-throughput cell lysis applications including DNA/ RNA extraction, protein extraction and pesticide residue analysis.

Programmable touch screen control panel for run time, rate, cycles and pause time.  Up to 500 protocols can be saved for recall.

Designed for vigorous up-and-down shaking of deep-well titer plates, vial sets, and centrifuge tubes.

Optional Kryo-Tech Accessories enable cryogenic grinding and preserve temperature sensitive samples for RNA and protein extractions.

Adjustable clamp with a release button that allows users to secure vials or titer plates of various sizes.

Typical sample processing time of 1-2 minutes.

Clamp Movement 1.25in (3.2cm) vertical

Clamp Speed Adjustable from 500-1750 strokes/minute

Timer Digital display in Minutes:Seconds (max 20:00)

USB port Run history is recorded and can be exported

Voltage 115V/60Hz or 230V/50Hz

CE Approved Yes

Dimensions 14.00 in (35.6 cm) wide x 22.5 in. (57.2 cm) deep x 28 in.(71.1 cm) high

Net Weight (lbs) 100 lbs (45.5kg)

Motor 1/3 hp

Power Cord 3-prong grounded plug, 115V 60Hz. 2-prong grounded plug, 230V 50Hz

Titer Plates & Cap Mats

2200 (2200) 96 - WELL TITER PLATE, SQUARE WELLS View Details

2200-100 (2200-100) 96 - WELL TITER PLATE, SQUARE WELLS, PACK OF 100 View Details

2201 (2201) CAP-MAT FOR 2200 TITER PLATE View Details

2201-10 (2201-10) CAP-MAT FOR 2200 TITER PLATE, PACK OF 10 View Details

2210 (2210) 96 - WELL TITER PLATE, ROUND WELLS View Details

2210-100 (2210-100) 96 - WELL TITER PLATE, ROUND WELLS, PACK OF 100 View Details

2211 (2211) CAP-MAT FOR 2210 TITER PLATE View Details

2211-10 (2211-10) CAP-MAT FOR 2210 TITER PLATE, PACK OF 10 View Details

2220-100 (2220-100) 48 - WELL TITER PLATE, RECTANGULAR WELLS, PACK OF 100 View Details

2221 (2221) CAP-MAT FOR 2220 TITER PLATE View Details

2221-10 (2221-10) CAP-MAT FOR 2220 TITER PLATE, PACK OF 10 View Details

2230 (2230) 24 - WELL TITER PLATE, SQUARE WELLS View Details

2230-100 (2230-100) 24 - WELL TITER PLATE, SQUARE WELLS, PACK OF 100 View Details

2231 (2231) CAP-MAT FOR 2230 TITER PLATE View Details

2231-10 (2231-10) CAP-MAT FOR 2230 TITER PLATE, PACK OF 10 View Details

Vials And Vial Sets

2240-PC (2240-PC) PRE-CLEANED 5 ML POLYCARBONATE VIAL SET View Details

2241-PC (2241-PC) 5 ML POLYCARBONATE VIAL WITH SCREW-ON CAP View Details

2250 (2250) PRE-CLEANED 15 ML SHORT POLYCARBONATE VIAL SET View Details

2251-PC (2251-PC) 15 ML SHORT POLYCARBONATE VIAL WITH SCREW-ON CAP View Details

2251C (2251C) CAPS FOR 15ML SHORT POLYCARBONATE VIAL View Details

2252-PC-30 (2252-PC-30) 15 ML POLYCARBONATE VIAL View Details

2253-PC-48 (2253-PC-48) 50ML POLYCARBONATE VIALS, 48 PK View Details

2254 2254 THICK-WALLED POLYCARBONATE JAR SET (SOLD IN PAIRS) View Details

2255 (2255) CRYOGENIC GRINDING VIAL PACK View Details

2300-500E (2300-500E) POLYPROPYLENE 2ML SKIRTED DISRUPTION TUBES AND CAPS View Details

2301-100MB (2301-100MB) PRE-FILLED 2ML TUBES W/ 100UM SILICA BEADS View Details

2302-1000AW (2302-1000AW) PRE-FILLED 2ML TUBES W/1.0MM ZIRCONIUM BEADS View Details

2302-1400AW (2302-1400AW) PRE-FILLED 2ML TUBES W/ 1.4 MM ZIRCONIUM BEADS View Details

2302-1700AW (2302-1700AW) PRE-FILLED 2ML TUBES W/ 1.7 MM ZIRCONIUM BEADS View Details

2302-200AW (2302-200AW) PRE-FILLED 2ML TUBES W/ 200UM ZIRCONIUM BEADS View Details

2302-3000AW (2302-3000AW) PRE-FILLED 2ML TUBES W/ 3.0 MM ZIRCONIUM BEADS View Details

2302-6000AW (2302-6000AW) 2ML PRE-FILLED VIALS - 6MM CERIA STABILIZED ZIRCONIA BEADS View Details

2303-MM1 (2303-MM1) PRE-FILLED 2ML TUBES WITH 500 UM GARNET AND A 6 MM ZIRCONIUM GRINDING SATELLITE View Details

2303-MM2 (2303-MM2) PRE-FILLED 2ML TUBES WITH 800 UM AND 1.4 MM ACID WASHED ZIRCONIUM BEADS View Details

2303-MM3 (2303-MM3) PRE-FILLED 2ML TUBES WITH 100 UM SILICA BEADS, 1.4 MM ZIRCONIUM BEADS AND A 4 MM SILICA BEAD, ACID WASHED View Details

2304-100AW (2304-100AW) PRE-FILLED 2ML TUBES W/ 100 UM SILICA BEADS (600MG) View Details

2304-400AW (2304-400AW) PRE-FILLED 2ML TUBES W/ 400UM SILICA BEADS FOR BACTERIA APPLICATIONS View Details

2304-800AW (2304-800AW) PRE-FILLED 2ML TUBES W/

800UM SILICA BEADS View Details

2305-2800SS (2305-2800SS) PRE-FILLED 2ML TUBES W/ 2.8 MM S/S GRINDING BALLS View Details

2310 (2310) 2ML DURATUBE View Details

Grinding Media

2100 (2100) GRINDING BALL DISPENSER View Details

2110M (2110M) 24-PIN MAGNET View Details

2110M-12 (2110M-12) PACK OF 12 MAGNETIC TIPS View Details

2110M-96 (2110M-96) PACK OF 96 MAGNETIC TIPS View Details

2110S (2110S) SEPARATOR, 24-PIN MAGNET View Details

2150 (2150) STAINLESS STEEL GRINDING BALLS, 5/32 IN. View Details

2155 (2155) STAINLESS STEEL GRINDING BALLS, 3/8IN View Details

2156 (2156) STAINLESS STEEL GRINDING BALLS, 7/16 IN. View Details

2160 (2160) SILICA GRINDING BEADS (800-1000 UM) View Details

2162 (2162) LOW BINDING SILICA GRINDING BEADS (800 UM) View Details

2165 (2165) SILICA GRINDING BEADS (400-600 UM) View Details

2166 (2166) SILICA GRINDING BEADS - MOLECULAR BIOLOGY GRADE View Details

2167 (2167) LOW BINDING SILICA GRINDING BEADS (400 UM) View Details

2168 (2168) LOW BINDING SILICA GRINDING BEADS (100 UM) View Details

2180 (2180) ZIRCONIUM GRINDING BEADS - MOLECULAR BIOLOGY GRADE View Details

2181 (2181) LOW BINDING ZIRCONIUM GRINDING BEADS View Details

2182 (2182) LOW BINDING ZIRCONIUM GRINDING BEADS View Details

2183 (2183) CERAMIC GRINDING CYLINDER, 3/8IN X 7/8IN View Details

2185 (2185) CERAMIC GRINDING CYLINDER, 5/32IN X 5/16IN View Details

Kryo-Tech®

2189C (2189C) CRYOPLATE View Details

2260 (2260) CRYO-BLOCK FOR 2255 CRYOGENIC GRINDING VIAL SET View Details

2600 (2600) CRYO-STATION View Details

2650 (2650) CRYO-ADAPTER FOR 2210 TITER PLATES View Details

2660 (2660) CRYO-BLOCK FOR 15 ML SHORT POLYCARBONATE VIALS View Details

2661 (2661) CRYO-BLOCK FOR 15 ML POLYCARBONATE CRYOVIALS View Details

2662 (2662) CRYO-BLOCK FOR 5 ML POLYCARBONATE VIALS View Details

2663 (2663) CRYO-BLOCK FOR 5 ML POLYETHYLENE VIALS View Details

2664 (2664) CRYO-BLOCK FOR 50 ML POLYCARBONATE CRYOVIALS View Details

2665 (2665) CRYO-BLOCK FOR 96 MICROCENTRIFUGE OR PCR TUBES View Details

2666 (2666) CRYO-BLOCK FOR 48 MICROCENTRIFUGE OR PCR TUBES View Details

Racks & Holders

2189T (2189T) NESTING TRAY SET View Details

2191 (2191) 5 ML VIAL HOLDER View Details

2193 (2193) 15 ML SHORT VIAL HOLDER View Details

2196-16-PE (2196-16-PE) FOAM HOLDER FOR 50 ML CENTRIFUGE TUBES View Details

2197 (2197) FOAM HOLDER FOR 15 ML CENTRIFUGE TUBES View Details

2300 (2300) FOAM HOLDER FOR 2ML TUBES View Details

Clamps

2011 2011 - ADJUSTABLE CLAMP FOR 2010 GENO/GRINDER View Details

2189 (2189) ADJUSTABLE CLAMP FOR ORIGINAL 2010 GENO/GRINDER View Details

2189G 2189G - ADJUSTABLE CLAMP FOR ORIGINAL 2010 GENO/GRINDER LARGE LID View Details

Tissue Homogenization and Cell Lysis

What are the applications for the Geno/Grinder®?

The SPEX SamplePrep 2010 Geno/Grinder® was originally designed as a titer plate shaker for extracting DNA from seeds and plant tissue. This involved shaking two sealed, 96-well titer plates with plant tissue, buffer, and a steel grinding ball in each well. Over the years, the versatility of the Geno/Grinder design has enabled it to accommodate a wider variety of sample containers and sample types. Not only can it be used for high-throughput tissue homogenization, DNA/RNA and protein extraction, it can also be used for lysing bacteria and yeast. Most recently it has been used to increase sample throughput for pesticide residue analysis using the QuEChERS method. A wide range of Application Notes are available which discuss the various Geno/Grinder® applications in more detail.

What are the advantages of the Geno/Grinder® over other tissue homogenizers?

Unlike other tissue homogenizers which clamp titer-plates on their side and move in a "figure-eight motion", the 2010 Geno/Grinder® uses a vertical shaking motion - a much more efficient grinding process. This ensures that the grinding media impacts the sample each and every time and ultimately results in higher yields. Another benefit of the vertical grinding action is that titer-plates and vials are kept in their upright position, eliminating potential well-to-well contamination. The Geno/Grinder is also able to accommodate a larger quantity and variety of sample formats including micro-centrifuge and PCR tubes, titer-plates, vial sets, and conical bottom centrifuge tubes. The list of Geno/Grinder accessories has been greatly expanded making it possible to grind a much wider range of samples. Cryo-Blocks® and related Kryo-Tech® accessories preserve temperature sensitive samples and can be used for extracting RNA and proteins from both plant and animal tissue.

What are the benefits of grinding at low temperatures?

Most samples become embrittled at low temperatures, making them easier to grind. When working with nucleic acids, grinding at low temperatures can also result in higher yields.

What type of samples can I process in the Geno/Grinder®?

The 2010 Geno/Grinder® can be used for a variety of samples including plant tissues like soybeans, sugar beet leaves, fungi, and seeds. It also grinds animal tissues like liver and fat and can lyse yeast and bacterial cells.

Grinding and Pulverizing

How to I choose a laboratory mill?

If your samples can be pulverized by impact at room temperature, you have a choice of several proven SPEX SamplePrep laboratory mills. The 8530 Enclosed Shatterbox® and 8500 Shatterbox® are ring-and-puck mills that are ideal for rapid grinding of samples up to approximately 100 grams, in 5 minutes or less. The 8000M Mixer/Mill® and 8000D Dual Mixer/Mill® are high-energy ball mills that not only pulverize samples in the 10 gram range but are suitable for blending powders and making emulsions up to approximately 50 mL. The 5100 Mixer/Mill® is a smaller high-energy ball mill, with about 10% the sample capacity of the 8000-series Mixer/Mills. All these mills have multiple-sample capacity with smaller samples, and a wide choice of grinding container sizes and materials, for maximum on-the-job flexibility over a wide rangeof applications. Soft, brittle materials and flexible samples that can be ground by shearing as well as impact may be suitable for the 5200 Micro Hammer-Cutter Mill, which uses revolving hammers in a chamber with a serrated lining. Product particle size is controlled by perforated screens in the bottom of the grinding chamber. This is the only "flow-through" mill sold by SPEX SamplePrep, and has proven useful for a comparatively narrow range of samples. The 6770 Freezer/Mill® and 6870 Freezer/Mill® are unique cryogenic mills that can grind almost any "ungrindable" sample, including all plant and animal tissue, most polymers, and countless other samples that are resistant to room-temperature milling. Freezer/Mills require liquid nitrogen for chilling the sample, and maintaining cryogenic temperatures in the vial during grinding. Forty years after its introduction the Freezer/Mill remains the most widely used and most effective cryogenic mill in the world. Finally, the 2010 Geno/Grinder® is a high-throughput tissue homogenizer with an adjustable clamp that accommodates a variety of formats ranging from deep-well titer plates to centrifuge tubes. It is specifically designed for rapid cell disruption, lysis, and tissue homogenization while preserving temperature sensitive samples. To discuss any SPEX SamplePrep laboratory mills and their suitability for your applications, feel free to contact our product specialists. We also offer test-grinding of your samples in our applications lab, or we can loan you a mill for short-term trials in your own lab. The more you know about SPEX SamplePrep laboratory mills, the more

How have the Freezer/Mills® been used in the field of medical research?

Medical schools and research hospitals have long used Freezer/ Mill technology in a variety of unconventional projects. One is the pulverizing of fresh, sterilized bone to use as a cement in joint-replacement surgery. The tiny bone fragments act as growth nuclei to stimulate the formation of bone around prosthetic implants. Another is the homogenizing of heart tissue to smear on slides as a culture medium. Freezer/Mills have even been used to grind implant materials to a fine powder which can be injected into live subjects for allergy and toxicity testing. At dental schools cryogenic grinding is used to pulverize fresh teeth without degrading them through heating. The overall possibilities in the medical field seem endless. A wide range of Application Notes which discuss the various Freezer/Mill® applications in more detail are available.